TB-500 Dosing Guide
A twice-a-week peptide with a loading phase, dosed in milligrams rather than micrograms.
TB-500 is the other half of the classic recovery pairing with BPC-157, but it is used very differently: the dose is about ten times larger and it is taken only once or twice a week. Nearly every guide describes a “loading” period of higher weekly totals followed by a lighter maintenance phase.
At a glance
- Typical dose
- 2–2.5 mg per injection (2.5 mg is the most-cited figure)
- How often
- Twice a week while loading, then once a week
- Route
- Subcutaneous, anywhere — it works systemically
- Timing
- Any time of day; fixed days (e.g. Monday / Thursday)
- How long
- 4–6 week loading phase, then 6–12 weeks maintenance; 4+ weeks off
- Vial size
- 10 mg
How much
The consensus is 2 to 2.5 mg per injection, with 2.5 mg the single most-cited number. The range across sources runs from 2 mg up to 5 mg per injection.
Weekly totals during the loading phase are 4–6 mg — most commonly 5 mg a week as 2.5 mg twice. During maintenance the weekly total drops to 2–2.5 mg.
How often & when
Loading: twice a week (for example Monday and Thursday) for 4 to 6 weeks. Maintenance: once a week, though some sources stretch this to once every 10–14 days.
Unlike BPC-157, TB-500 does not need to be injected near the injury — it circulates. Abdomen, thigh or upper arm are all used; some people inject it intramuscularly, which sources treat as acceptable but not necessary.
How long
The typical course is 4–6 weeks of loading plus 6–12 weeks of maintenance, or a fixed 8–12 week cycle, followed by 4–8 weeks off. Four weeks off is the most common break.
The reason the schedule is so sparse is the half-life: sources quote 2–3 days, which is why twice-weekly dosing is considered enough.
Mixing the vial
Doses below are for a standard U-100 insulin syringe (100 units = 1 mL). The formula: units = dose in mg ÷ concentration in mg/mL × 100. Bacteriostatic water is the usual diluent. A standard peptide vial holds about 3 mL of liquid at most — the store’s vials included — so every row below stays at or under 3 mL. Guides elsewhere that call for 4–5 mL will not fit.
| Vial | Water added | Concentration | Doses in units |
|---|---|---|---|
| 10 mg | 2 mL | 5 mg/mL | 2 mg = 40 units · 2.5 mg = 50 units · 4 doses of 2.5 mg per vial |
| 10 mg | 3 mL | 3.33 mg/mL | 2.5 mg = 75 units |
At 2.5 mg twice a week, one 10 mg vial is two weeks of loading or four weeks of maintenance. The 0.5 mL injection volume is larger than most peptides — rotate sites.
How to reconstitute
Not sure how to mix the vial? Our free Reconstitution Calculator walks you through it: enter the vial size, how much bacteriostatic water you're adding and the amount you want per dose, pick your syringe, and it tells you exactly what mark to draw to, the concentration, and how many doses the vial holds — with a syringe diagram and a step-by-step worked example.
Practical tips
- Refrigerate after mixing and use within 28–30 days. Do not freeze the mixed solution.
- Because each shot is a relatively large volume (half a syringe at the 2 mL mix), rotating sites keeps irritation down.
- Commonly stacked with BPC-157: BPC-157 daily plus TB-500 twice a week. The store’s BB blend combines them 1:1 in one vial — that changes the math, so read the BB guide if you use the blend.
- Pick fixed injection days and keep them; the schedule is easy to lose track of at once or twice a week.
What the evidence looks like
Evidence level
There is no published human trial of the injectable TB-500 fragment. Human data exists only for the full-length thymosin beta-4 protein (topical and eye-drop studies, plus early IV safety work). The dosing here is community convention extrapolated from animal studies — there is no validated human dose.
For what the compound is and how it is studied — mechanism, research history and the primary literature — read the reference entry: TB-500.
Batch-tested with published COAs
TB-500 on the store
Every vial is tested by an independent lab, with a batch-linked certificate of analysis you can check before you buy. This page is educational; purchasing happens on the store.
Common questions
How many units is 2.5 mg of TB-500?
50 units on an insulin syringe when 10 mg is mixed in 2 mL of bacteriostatic water (5 mg/mL).
Why load first?
It is the near-universal convention in the sources: 4–6 mg a week for 4–6 weeks builds up levels, after which a single weekly dose is thought to hold them. There is no human trial behind the pattern.
Does it have to go near the injury?
No. Sources consistently describe it as systemic — abdomen, thigh or upper arm all work.
Sources
The figures above were compiled from the following pages. Where an FDA label exists it is listed first; the rest are vendor, clinic and community dosing references, which are not peer-reviewed.
- TB500.org — Dosage — 2–2.5 mg twice weekly loading 4–6 weeks; once weekly maintenance; systemic; use within 28–30 days Open
- Peptide Dosing Protocols — TB-500 — 4–5 mg/week loading; 8–12 week cycle, 4–8 weeks off; 50 units = 2.5 mg Open
- Peptide Deck — TB-500 dosage guide — 4–6 mg/week split twice weekly; 4-week washout Open
- Path to Peptides — TB-500 protocol — 2.5 mg Mon/Thu weeks 1–6, then weekly; no validated human dose Open
- Peptide Initiative — TB-500 — 2–2.5 mg twice weekly; half-life 2–3 days Open