NAD+ Dosing Guide
50–100 mg under the skin, a few times a week, injected slowly because it stings.
NAD+ is a coenzyme rather than a peptide, used for energy and healthy-aging goals. Injected doses are far larger than peptides — tens of milligrams — and there are two things everyone mentions: inject it in the morning, and inject it slowly, because the sting is real. Clinics also give it by IV drip at much higher doses; that is a different setting.
At a glance
- Typical dose
- 50–100 mg per injection (start at 25–50 mg)
- How often
- 2–3 times a week, or daily / every other day
- Route
- Subcutaneous (also intramuscular); IV infusions are a clinic thing
- Timing
- Morning — evening doses are linked to insomnia
- How long
- 8–12 week course, then a lighter maintenance schedule; no strict cycling
- Vial size
- 100 mg
How much
The consensus subcutaneous dose is 50–100 mg per injection, with 100 mg the most-cited target and 50 mg the usual starting point. The range runs from 25 mg (a cautious first week) up to 200–250 mg per injection in advanced protocols.
For context, IV clinics infuse 250–500 mg as a standard session and 500–1,000 mg as a loading dose, over two to four hours. Conservative clinic protocols cap intramuscular injections at 100 mg. None of that transfers to a syringe at home.
How often & when
Two patterns split the sources about evenly: 50–100 mg two or three times a week, or 50–100 mg daily to every other day. Typical weekly totals are 150–300 mg; one reference suggests treating 300 mg a week as a ceiling.
Morning dosing is recommended consistently, because evening injections are associated with trouble sleeping. Ramping is standard — for example 25 → 50 → 100 mg over four or five weeks, or 50 → 75 → 100 mg over three weeks.
How long
Sources agree NAD+ does not need strict cycling — it is used by the body as a substrate rather than acting on a receptor that adapts. Typical courses are 8–12 weeks (some 8–16), followed by a taper to one to three injections a week, or a switch to oral precursors (NMN or NR) as a bridge.
Mixing the vial
Doses below are for a standard U-100 insulin syringe (100 units = 1 mL). The formula: units = dose in mg ÷ concentration in mg/mL × 100. Bacteriostatic water is the usual diluent. A standard peptide vial holds about 3 mL of liquid at most — the store’s vials included — so every row below stays at or under 3 mL. Guides elsewhere that call for 4–5 mL will not fit.
| Vial | Water added | Concentration | Doses in units |
|---|---|---|---|
| 100 mg | 1 mL | 100 mg/mL | 1 mg per unit: 25 mg = 25 u · 50 mg = 50 u · 100 mg = 100 u (the whole vial) |
| 100 mg | 2 mL | 50 mg/mL | 50 mg = 100 u; a 100 mg dose becomes two 1 mL injections at different sites |
The 100 mg/mL mix matches the concentration of pre-mixed pharmacy NAD+ and keeps the math to 1 mg per unit. A 100 mg vial is one full dose or two 50 mg doses. If you buy pre-mixed liquid, use the concentration on the label.
How to reconstitute
Not sure how to mix the vial? Our free Reconstitution Calculator walks you through it: enter the vial size, how much bacteriostatic water you're adding and the amount you want per dose, pick your syringe, and it tells you exactly what mark to draw to, the concentration, and how many doses the vial holds — with a syringe diagram and a step-by-step worked example.
Practical tips
- Stinging or burning at the site for 5–15 minutes is near-universal. What helps: inject slowly (10–30 seconds), let the vial reach room temperature, dilute more, ice the site, or split the dose across two sites.
- Fast injection also brings flushing, nausea and chest tightness — all rate-dependent, all avoided by going slowly.
- Start at 25–50 mg and work up; headache, fatigue and nausea are dose-related.
- Refrigerate, protect from light, and use a mixed vial within 2–4 weeks. Pre-mixed product has its own use-by date.
What the evidence looks like
Evidence level
Human trial evidence supports only the oral precursors (NMN, NR) and some small IV safety and pharmacokinetic studies. Subcutaneous and intramuscular dosing, and the IV clinic protocols, are clinic and community convention rather than trial-derived.
For what the compound is and how it is studied — mechanism, research history and the primary literature — read the reference entry: NAD+.
Batch-tested with published COAs
NAD+ on the store
Every vial is tested by an independent lab, with a batch-linked certificate of analysis you can check before you buy. This page is educational; purchasing happens on the store.
Common questions
Why does NAD+ sting so much?
It is an acidic, concentrated solution. Slow injection, warming the vial, diluting to 50 mg/mL and splitting the dose across two sites are the fixes every source repeats.
How many units is 50 mg?
50 units at 100 mg/mL (100 mg + 1 mL); 100 units at 50 mg/mL (100 mg + 2 mL).
Can I take it at night?
Sources advise against it — evening dosing is linked to insomnia. Morning is the standard.
Sources
The figures above were compiled from the following pages. Where an FDA label exists it is listed first; the rest are vendor, clinic and community dosing references, which are not peer-reviewed.
- The Peptide Catalog — NAD+ dosing guide — 100 mg 2–3×/week (50 mg start); 100 mg + 1 mL = 100 mg/mL; 8–12 weeks Open
- Form Blends — NAD+ how to use — 50–100 mg daily to 3×/week; inject over 5–10 s Open
- Peptide Dosing Protocols — NAD+ — SubQ 50–100 mg 2–3×/week; IV 250–1,000 mg; morning; ~300 mg/week cap Open
- Peptides Insider — NAD+ dosage — 50–100 mg (up to ~250 mg); no cycling needed; sting mitigation Open
- Olympia Pharmacy — NAD+ dosage chart — 503B compounder: 100 mg/mL product; 30 mg daily ramp then 50 mg Mon–Fri; IV protocols Open
- Peach IV — NAD+ dosage & frequency guide — 25 → 50 → 100 mg ramp; IV 500–1,000 mg loading; 8-week protocol Open